Numerical simulations of rheological conditions and cell motion in chamber entrance. (A) Maps of shear rates at chamber bottom wall for Cellix chamber for flow of 4 µL/min (left panel) and for Ibidi chamber for flow of 10 µL/min (right panel) corresponding experimentally to a desired shear rate of 100 s⁻¹ in the observation section of the chamber. Scale bars, 200 µm. (B) Map of fluid and cell velocity in a sagittal cut of the Cellix chamber (left panel) or the Ibidi chamber entrance (right panel) along its symmetry axis. Cells are simulated as spheres. (C) Number of deposited cells at t = 60 seconds as a function of the cell concentration perfused into the chambers. (D) Representative images of fluorescently labeled neutrophils (Ly6G, pink) and extracellular DNA (exDNA, green) after perfusion (100 s⁻¹ shear rate) on VWF in a Cellix flow chamber (n = 3). Scale bars, 100 µm. Dashed lines have been added to delimit the flow channels and the well entrance.