Epitope-specific immunoblotting. (A-B) Representative immunoblots depicting annexin A2, S100A10, annexin A5, and GAPDH expression in PBMCs. PBMC lysates from HCs as well as subjects with VTE and low (labeled in red) or normal A2 expression were probed with IgG directed against the core domain of A2, as well as IgGs directed against S100A10, GAPDH, and annexin A5. A sample from the same internal control (IC) was also examined in each blot. FH status, presence or absence of an IT, and presence or absence of the 5-SNP cluster are also indicated. Black dots indicate not tested. All results shown are representative of blots on samples collected on ≥2 separate occasions ≥3 months apart. L, low; N, normal.