FCGR1A (CD64) expression on AML increases its sensitivity to DNTs. (A) CD64 expression in KG1a cells transduced with FCGR1A or a control vector. (B) Killing of the control or FCGR1A-overexpressing KG1a cells by DNTs at a 1:1 E:T ratio. This experiment was repeated 3 times with similar results, and a 2-tailed Student t test was used to examine differences. (C) NSG mice (4-5 per group) were engrafted with control or FCGR1A- overexpressing KG1a cells, followed by treatment with 3 doses of DNTs or phosphate-buffered saline (with interleukin-2) on days 1, 4, and 7 postinoculation of leukemic cells. Mice were euthanized when bone marrow engraftment of KG1a cells reached ∼70%. A 2-tailed Student t test was performed to determine statistical differences. (D) Eleven primary AML samples were cocultured with DNTs for 2 hours at an E:T ratio of 4:1 in triplicates. Scatterplot depicts the Pearson’s correlation coefficient for the mean killing of primary AML against the proportion of CD64⁺ cells. (E-F) In vitro cytotoxicity assay conducted against 3 primary AML samples (090596, 110010, and 130794) with CD64⁺ and CD64⁻ leukemic blast populations at a 4:1 E:T ratio. (E) Representative flow plot showing gating strategy for leukemic subpopulations with or without CD64 expression. (F) Representative percentage of specific killing induced by DNTs from 1 donor. Two-tailed Student t tests were performed to detect statistical differences between the CD64⁻ and CD64⁺ populations within each patient sample. This experiment was performed using DNTs expanded from 3 donors. **P < .01, ***P < .001, ****P < .0001.