Characterization of PC neoplasm in KO mice. (A-B) Histologic and IHC stains of indicated markers on serial LN (A) and BM (B) sections from age-matched WT control and 2 representative KO mice with EP. Scale bars: white, 100 µm; black, 20 µm. (C) Bone radiographs of limbs with muscles still attached (top) or after removal (bottom) of WT and KO mice with PC neoplasm. One representative bone radiograph from each group is magnified to facilitate the interpretation. Note absence of bone lytic lesions in all EP cases. (D) Serum protein electrophoresis of WT and KO mice with PC neoplasm. Note presence of M spike in 1 of the EP cases. (E) Plasma IgM and IgG concentrations assessed using ELISA in WT and KO mice with PC neoplasm. Graphs depict the mean plus or minus SD. (F-G) miR-15a and miR-16 expression (ISH) assessed on FFPE biopsy sections from patients with EP (n = 11). (F) The ISH signal was scored into 3 grades ranging from negative, to weak, to strong in comparison with healthy nodal PCs. (G) A representative example for each grade is shown. Scale bar, 50 µm.