Molecular identity of tumor cells in Rosa26^HGAL/Sca1-Cre mice. (A) Heat map of genes significantly induced or repressed in Rosa26-^HGAL/Sca1-Cre splenic tumors (n = 4) and WT spleens (n = 3) classified by 226 genes/probe sets (FDR = 0.101) that are differentially expressed between tumor and WT spleens. Each row represents a separate gene, and each column denotes a separate messenger RNA (mRNA) sample. The level of expression of each gene in each sample is represented using a red–blue color scale (upregulated genes are displayed in red, and downregulated genes are in blue). (B-D) GSEA of the transcriptional signatures within splenic tumors compared with control spleen from WT littermates. Gene expression data from Rosa26-^HGAL/Sca1-Cre tumors showed significant enrichment in genes upregulated in follicular lymphomas described by Shipp et al⁴¹  (GSEA FDR q value = 0.000) (B), enrichment in MYC target genes (GSEA FDR q value = 0.000) (C), and significant enrichment in genes expressed in normal GCBs (GSEA FDR q value = 0.000) (D). (E) Heat map of genes significantly induced or repressed within Rosa26-^HGAL/Sca1-Cre splenic tumors (n = 4), Rosa26-^HGAL/Sca1-Cre spleens without tumors (n = 3), Rosa26-^HGAL/Mb1-Cre spleens (n = 5), Rosa26-^HGAL/Aid-Cre spleens (n = 4), and WT spleens (n = 3) classified by 226 genes/probe sets (FDR = 0.101) that are differentially expressed between Rosa26-^HGAL/Sca1-Cre splenic tumors and WT spleens. Each row represents a separate gene, and each column denotes a separate mRNA sample. The level of expression of each gene in each sample is represented using a red–blue color scale (upregulated genes are displayed in red, and downregulated genes are shown in blue). NES, normalized enrichment score.