In DLBCL tumors, PD1⁺TIM3⁺T cells are exhausted. CellTrace violet–labeled mononuclear cells from DLBCL (n = 13) and control group (n = 12 [rLN, n = 3; cHL, n = 6; FL, n = 3]) tissues were activated by plate-coated CD3 and soluble CD28 antibodies. (A) Proliferative capacity of CD4⁺ and CD8⁺ T cells, determined by the percentage of CellTrace^dim cells and the concentration of IFN-γ detected in the culture supernatant. (B) Correlation between the percentage of PD1⁺TIM3⁺ CD4⁺ or CD8⁺ T cells and their proliferation and IFN-γ secretion (n = 18 or 20 DLBCL). (C) Correlation between the percentage of proliferated T cells and the concentration of IFN-γ detected in the supernatant (n = 18 or 20 DLBCL;GC, blue; ABC, red; unknown, green). (D) Correlation between percentage of CD4⁺ or CD8⁺ T cells coexpressing PD1 and TIM3 and their intracellular IFN-γ or granzyme B secretion (n = 12; GC, blue; ABC, red; cell of origin not defined, green).