Longitudinal RIS analysis. All unique RISs identified from (A) X348, (B) X349, (C) R2202, and (D) R2203 are plotted based on the number of times the RIS was sequenced (relative sequence capture) and normalized to the percentage of total RISs captured at each time point for each dog. The gray portion of each bar represents the percentage corresponding to all but the top 10 most frequently captured RISs. The 10 most frequently captured RISs in each sample are represented by the white boxed lower portions of each bar graph. RISs identified at both time points in the same animal were shaded in matching colors in the bar representing each time point. The gold asterisk in panel B represents the position of a clone identified 19 days postinjection that was also sequenced at 112 days postinjection but is impossible to visualize due to the small frequency of this RIS at 19 days postinjection.