![Effects of SMOC1 on thrombin-dependent responses in murine platelets. (A) Thrombin-induced phosphorylation of β3-integrin (on Tyr773; pY773) in platelets from WT and SMOC1+/− (+/−) mice; n = 5 animals per group. **P < .01, ANOVA and Bonferroni test. (B) Effect of thrombin (Thr; 0.1 U/mL) on β3-integrin phosphorylation (on Tyr773; pY773) in platelets from SMOC1+/− (+/−) mice pretreated with solvent (Sol) or murine recombinant SMOC1 (rSMOC1; 10 µg/mL, 45 minutes); n = 8 per group. ***P < .001, 2-way ANOVA and Bonferroni test. (C) PAR3 and PAR4 receptor expression in platelets from WT and SMOC1+/− (+/−) mice; n = 4 animals per group. (D) Basal and agonist-induced increases in platelet Ca2+ levels ([Ca2+]i) in platelets from WT and SMOC1+/− (+/−) littermates stimulated with thrombin (Thr; 1 U/mL), a PAR3 agonist (100 µM), or a PAR4 agonist (100 µM); n = 4 animals per group. ***P < .001, 2-way ANOVA and Bonferroni test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/137/12/10.1182_blood.2020009405/1/bloodbld2020009405f2.png?Expires=1722699115&Signature=qrrKO7hSBpc8tTm5EvSy4PTdg3twa7GCkgJO2IFXPRJj8BGqI1BazdBfFUTqVUdQQo~7kQYLSdIkdCHCRbK301~mmR56-O-0vw-fff-iJqQf-X8LOxezIzZR4K0uEYbuLP8OlS77aqA6fUp3SXpsFh3nZKgmobFyi17tZsE4tUOH6bCkIgKsmksbDvAktYILzu0yzhZxcKJUd-TMGOLDPDccAOCgMt430RvKjQg3oj9TVMaCQsWiRqeUCyTP-A~VLUAwb2xKlm9pe3rBZD4Ih-gUB65w2rtkSjN6hczN0B-1ycHySQru4b0Bt6L15pPxiIAhvv-X6uelpd9HuZmRtw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of SMOC1 on thrombin-dependent responses in murine platelets. (A) Thrombin-induced phosphorylation of β3-integrin (on Tyr773; pY773) in platelets from WT and SMOC1+/− (+/−) mice; n = 5 animals per group. **P < .01, ANOVA and Bonferroni test. (B) Effect of thrombin (Thr; 0.1 U/mL) on β3-integrin phosphorylation (on Tyr773; pY773) in platelets from SMOC1+/− (+/−) mice pretreated with solvent (Sol) or murine recombinant SMOC1 (rSMOC1; 10 µg/mL, 45 minutes); n = 8 per group. ***P < .001, 2-way ANOVA and Bonferroni test. (C) PAR3 and PAR4 receptor expression in platelets from WT and SMOC1+/− (+/−) mice; n = 4 animals per group. (D) Basal and agonist-induced increases in platelet Ca2+ levels ([Ca2+]i) in platelets from WT and SMOC1+/− (+/−) littermates stimulated with thrombin (Thr; 1 U/mL), a PAR3 agonist (100 µM), or a PAR4 agonist (100 µM); n = 4 animals per group. ***P < .001, 2-way ANOVA and Bonferroni test.