WTAP-mediated regulation of HK2 depends on its m6A methylase activity. (A) Relative mRNA levels of HK2 from qPCR analysis in IGF2BP knockdown cells compared with control cells. (B) SU-DHL-8 or Farage cells were transiently transfected with IGF2BP2-siRNA or siRNA control. The half-life of HK2 mRNA was measured. (C) Dual-luciferase reporter assays showed the effect of IGF2BP2 on HK2 reporters with either WT or MUT binding sites. (D) Schematic diagram depicting the mechanism by which piRNA-30473 contributes to tumorigenesis and poor prognosis in DLBCL by regulating m6A RNA methylation and thereby triggering corresponding signaling cascades. Error bars represent the mean ± SD of 3 independent experiments. ***P < .001; ****P < .0001.