WTAP is a functionally important target gene of piRNA-30473 in DLBCL. (A) Representative immunohistochemical staining (original magnification ×40) of WTAP in DLBCL patients is shown. WTAP-positive particles (brown) are apparent in the nucleus (nuclei stained with hematoxylin, blue). Original magnification ×40. (B) OS was plotted and compared using the Kaplan-Meier method and log-rank test. WTAP was dichotomized at the median based on low vs high expression. (C) SU-DHL-8 cells (si-NC or si-WTAP-transfected) were harvested at 24, 48, and 72 hours after transfection. Cell proliferation was assessed by CCK-8 assays. (D-E) Cell cycle distribution of control and WTAP-depleted SU-DHL-8 cells examined by DNA content index at 48 hours after transfection. (F) The percentage of apoptotic cells in control and WTAP-depleted SU-DHL-8 cells was examined at 48 hours after transfection. Error bars represent the mean ± SD of 3 independent experiments. ****P < .0001.