Treg-specific SPARC deficiency delays clot resolution and intrathrombic monocyte differentiation. RAG^−/− mice received 1 × 10⁶ splenic WT or SPARC^−/− Tregs and 5 consecutive IL-2/JES 6-1 complex injections. IVC ligation was performed 5 days after the last injection and thrombus formation/resolution followed by ultrasound. (A) Ultrasound data of thrombus resolution. (B-D) Representative monocyte numbers and phenotypes in WT or SPARC^−/− Treg recipients at day 21. Results are shown as mean ± SEM (n = 3/group; ***P ≤ .0005). Statistical differences were tested using an unpaired Student t test (2 tailed). Results are representative of 3 independent experiments.