Iron restriction improves hematological parameters in Townes sickle mice. (A-E) Townes HbSS and trait HbAS mice weaned and maintained on either a 48- or 20-ppm iron diet for 8-10 weeks. Then serum iron (A), mean corpuscular hemoglobin concentrations (B), red blood cell counts (C), hematocrit (D), hemoglobin concentration (E), and reticulocyte count (F) were measured; n = 6-13 mice per group. Data represented as means ± standard error of the mean. *P < .05; ***P < .005; ****P < .001. ns, nonsignificant.