Impaired mobilization in FGF-23^−/− mice. (A) Efficiency of G-CSF mobilization as assessed by white blood cells (WBCs), LSKs, and CFU-Cs in the blood in FGF-23^+/+ and FGF-23^−/− mice (n = 3 for the PBS/BSA group; n = 6-7 for the G-CSF group). (B-D) Assessment of competitive repopulating activity in G-CSF mobilized blood from FGF-23^+/+ or FGF-23^−/− mice. Competitive repopulation assay (B), %CD45.2 level in leukocyte chimerism (C), and RUs at 6 months after transplantation (D) (n = 5). Combined data of at least 3 independent experiments are shown. Data are means ± SEM. ***P < .001 (Student t test).