SRP54-mutant alleles impair granulocytic differentiation of CD34⁺ cord blood cells. (A) Schematic overview of the experimental setup for isolation, lentiviral transduction, and in vitro cultivation of CD34⁺ cord blood cells followed by flow cytometric analyses. (B) Gating strategy to identify CD15 and CD16 double-positive neutrophils. (C) Histograms of flow cytometric analyses of CD11b levels of SRP54 transduced and differentiated CD34⁺ cells. (D) Flow cytometric quantification of CD11b expression of transduced and differentiated CD34⁺ cells. Plot shows the geometric mean fluorescence intensity of WT and G226E transduced cells. A ratio paired Student t test was used for statistical analysis. G-CSF, granulocyte colony-stimulating factor; IL-3, interleukin 3; SCF, stem cell factor. *P < .05. Bar plots represent the mean value of the replicates. Error bars indicate the standard deviation of the mean.