SDS-agarose and SDS-PAGE of VWF multimers. (A) SDS–agarose electrophoresis gels of VWFm in the patient, his wife, and daughter. The bands were detected with a ChemiDoc densitometer (Bio-Rad). (B) Discontinuous high-resolution (0.8% to 2.5%) SDS-agarose gel electrophoresis of VWFm from the same plasma sample. (C) Western blots of VWF samples of the patient reduced with phosphine (Bio-Rad) in precast SDS-PAGE (4% to 12%) gel using a Criterion apparatus (Bio-Rad).The arrows indicate VWF bands produced by the ADAMTS-13 proteolysis. NP, normal plasma.