Autophagy deficiency exerted differential effects on hematopoiesis between adult and neonatal mice. (A) Cell division activity were determined by EdC uptake. Histograms represent EdC⁺ cells, and graphs show the frequency of EdC⁺ cells. Data are presented as means ± SD (n = 3 in each condition, 2 independent experiments; *P < .05 by Student t test). (B) To examine mitochondrial status, ΔΨm was also assessed. Histograms represent the fluorescent intensity of indicated dyes, and graphs show relative values against Atg7^fl/fl-derived HSCs. Data are presented as means ± SD (n = 3 in each condition, 2 independent experiments; *P < .05 by Student t test). (C-F) Total number of BM cells (P5 = whole body, 4 and 8 weeks = both tibias and femurs) (C), the frequency of HSCs (D), the frequency of LEK cells (Lineage⁻, EPCR⁺, and c-Kit⁺) (E), and the number of white blood cells (F) in Atg7^fl/fl and Atg7^fl/fl; Vav-Cre mice were examined at P5, 4 weeks, and 8 weeks old. Data are presented as means ± SD (n = 3 in each condition, 2 independent experiments; *P < .05 by Student t test). (G) Cell cycle status was also determined by the combination between Hoechst 33342 and Ki67. Graphs show the cells with indicated cell cycle phase. Data are presented as means ± SD (n = 3 in each condition, 2 independent experiments; *P < .05 by Student t test).