Reclassifying a T-ALL iu BPDCN by gene expression profiling. (A) Unsupervised hierarchical clustering of 176 AL patients and 10 normal DC transcriptomes: B-ALL (green), T-ALL (yellow), AML (lavender), BPDCN (magenta), reclassified patient P13 (yellow with gray dot), normal cDC (red), and normal pDC (light blue). (B) Flow cytometry analysis of patient P13 BM: blastic population (magenta) and CD45⁺ lymphocytes (light green). The blastic population expresses CD45^low cCD3^low and CD4⁺, CD2⁺, CD10⁺ but no CD7 and surface CD3. The blastic population expresses the pDC markers CD123, TCL1^high and a low level of CD304. CD303 and CD56 were not expressed. (C) BM examination of patient P13 showed blasts, with regular nuclei and small nucleoli, no cytoplasmic granulation, and rare small vacuoles and/or pseudopodia (May-Grünwald-Giemsa stain, magnification ×1000).