RUNX1 mutations detected in the HOVON102 cohort of AML patients. (A) Variant allele frequencies (VAFs) of all RUNX1 mutations in the entire HOVON102 cohort at diagnosis (n = 142 in 115/763 patients), VAFs of all RUNX1 mutations in the subcohort of patients who attained complete remission (CR), and for whom paired samples were available, at diagnosis and at remission (n = 48 in 37/287 patients). (B-D) VAFs of all mutations of patients no. 748, no. 124, and no. 740, at diagnosis and during remission. (E) Visualization of the 48 RUNX1 mutations identified in the 37 paired samples from CR patients, annotated to NM_001754.5 (supplemental Table 2). *, stop codon; (2), identical variant is identified in 2 different patients; Gln/Glu, the nucleotide at this location is mutated in 2 patients, changing to Gln and Glu, respectively; indel, insertion or deletion; RUNT, runt homology domain; TAD, transactivation domain.