Changes in glycan binding observed among healthy, SCT, and SCD plasma samples using glycan microarrays. (A) GBP binding to 100 different glycan structures was measured using Glycan Array (RayBiotech). Glycans are split into structural/functional groups based on numbering. (B) Data obtained from healthy, SCD, and SCT patients were normalized using a quantile method and analyzed using a sparse partial least squares discriminant analysis (sPLS-DA) method. All glycan structures are listed with numerical classification in supplemental Figure 2 with additional contributing glycans to component 1 and 2 of separation shown in supplemental Figure 3. The receiver operating characteristic values are as follows: healthy vs others = 0.827; SCT vs others = 0.6526; SCD vs others = 0.9729. (C) Data showed significant differences in SCD compared with healthy and SCT groups (P ≤ .05) with log₂ fold changes between 2 and −2 (95% CI bars shown; for full t test results, see supplemental Table 7). Glycan structures for each numerical classification are shown with linkage information. Disease state preferences for each glycan are also indicated. Glycan names can be found in supplemental Table 6.