Mass spectrometry comparison of RBC N-glycans from healthy and SCD patients. Volcano plot of quantified RBC N-glycans with thresholds (shading) applied for significance (P ≤ .05) and fold change (at least twofold) between control (n = 3) and SCD donors (n = 3). Colored data points are glycans selected for display left and right from the plot. (A) N-glycan structures that are significantly increased in healthy patients. (B) N-glycan structures that are significantly increased in SCD.