Patients receiving high doses of iTregs have higher levels of Foxp3⁺CD25⁺cells in PB. PB was collected from patients on days 3, 7, 14, and 28 following adoptive transfer of rapa/TGFβ iTregs. After Ficoll, the percentage of of CD4⁺ cells that were Foxp3⁺CD25⁺ Tregs present in PBMCs was determined by flow cytometry. Low iTreg samples (n = 2) were from patients receiving ≤30 × 10⁶/kg, high iTreg dose (CSA only) was from patients receiving 300 × 10⁶ iTreg with CsA immunosuppression (n = 10) and high iTreg dose (sirolimus only) was from patients receiving 300 × 10⁶ iTreg with sirolimus immunosuppression (n = 2).