Assessing the phenotype and function of infused products. To assess the phenotype and function of clinical rapa/TGFβ iTreg products, aliquots were taken postprocessing and frozen. Frozen aliquots were then batch-analyzed for phenotype and function. Representative example (A) and summary (B) of Foxp3 expression in clinical iTreg products. (C) Expression of the suppressive molecule CTLA4 on clinical iTreg products. (D) Representative example and summary of 1:2 (Treg-to-PBMC) ratio for the indicated 9 clinical products. N, none; R/T, rapa/TGFβ.