Mutational landscape of the entire cohort. Proportion of patients with ≥1 mutation per gene according to the classification of mutations (A), the type of myelofibrosis (B), and the driver mutation (JAK2 or CALR) (C). The distribution of allelic burden of additional mutations is represented by violin plots (D), and the association between mutations on the genes of the 4-tier classification is shown in a circos plot (E). *P < .05; **P < .01; ***P < .001. Only pathogenic and likely pathogenic mutations were retained for the circos plot. VAF, variant allele frequency.