Aberrant proteome of neutrophils in CLL. Neutrophils from the BM and blood of non-CLL and CLL-bearing mice were isolated by cell sorting, and the proteome was analyzed by LC-MS/MS. (A-B) Receiver operating characteristic curves based on proteome of BM (A) and blood (B) neutrophils indicate alterations in non-CLL and CLL conditions. (C-D) Volcano plots illustrating fold change (log₂, x-axis) vs FDR-adjusted P value (−log₁₀ [FDR], y-axis) of the neutrophil proteome in the BM (C) and blood (D). The gray lines indicate the proteins that have passed a significance value of <.01 (−log₁₀ [FDR] >2). (E-F) Heatmaps of significant proteins from the volcano plots in panels C and D with an FDR-adjusted P < .01. Expression values of all proteins are shown in supplemental Tables 2 and 3. (G) Principal-component analysis of the significant proteins (FDR-adjusted P < .01) in the BM and blood indicate alteration of the neutrophil proteome in the blood of CLL-bearing mice. BM: non-CLL, n = 9; CLL, n = 13; blood: non-CLL, n = 6; CLL n = 12.