Increased acute infection of the urinary bladder in CLL. (A) Urinary bladders of CLL mice with a tumor burden of >20% and non-CLL mice were infected with UPECGFP, and the bacterial burden was determined on day 1 postinfection by fluorescence microscopy. The dashed lines indicate the border of the urothelium to the lumen and connective tissue. DAPI is shown in white and UPECGFP in green. Scale bars, 200 µm (left panel) and 50 µm (right panel). (B) Quantification of the microscopic images shown in panel A. The bacterial burden was calculated as UPEC-positive area in the indicated bladder tissues (lumen, urothelium, and connective tissue) in UPEC-infected non-CLL and CLL-bearing mice. **P < .01, ****P < .0001 (n = 6). Data represent mean ± SEM. ct, connective tissue; lu, lumen; uro, urothelium.