Thrombus resolution in mice with fibroblast-specific TGF-β overactivity. (A) Workflow for thrombus induction and (B) qPCR analysis for TGFB1 expression in lungs of control and TBRIIΔk mice at baseline (n = 4 mice per group). (C) Representative HFUS images showing thrombus area (left) and length (right) measurement on day 7 after ligation in control and TBRIIΔk mice. (D) Thrombus length, (E) thrombus volume as measured by HFUS (n = 5 mice per group), and (F) harvested thrombus weight (n = 5-8 mice per group). (G) Representative trichrome staining of mouse thrombus at different time points after ligation and collagen area represented relative to total thrombus area (n = 5-7 mice per group). Right: bar graph quantification for both groups is shown next to histochemistry panels (scale bars, 200 µm). (H) qPCR analysis of mouse thrombus at different time points (n = 4-6 mice per group). Data are shown as mean ± SEM. *P < .05; **P < .01 (unpaired Student t test).