CD34/CD38 LCS-phenotype of pediatric AML samples and pediatric hematopoietic progenitors. (A) While 6 AML CD45^dim populations show a very heterogenous CD34 vs CD38 distribution (top panel), their healthy counterparts show a relatively consistent distribution in 6 different cases. (B) Frequency of CD34⁺CD38⁻ cells in the CD45^dim gate in AML patients (median, 10.2%) and healthy donors (median, 4.5%). (C) PCA illustrating 4 populations: bulk AML (AML), AML CD34⁺CD38⁻ (AML LSC), healthy CD45^dim, and healthy CD45^dimCD34⁺CD38⁻. While both healthy populations cluster together, all 6 AML LSC samples cluster quite close to their corresponding bulk AML sample (as linked by a black line). PC, principal component.