Inhibition of BCL6 in AML cells resulted in regulations of multiple numbers of biologically important pathways. Three primary AML cells (AML37, AML95, and AML98) were treated with FX1 using the concentration for EC50 in pilot ex vivo treatment experiments, for 12 hours. RNA was extracted from dimethyl sulfoxide (DMSO)- or FX1-treated cells and subjected to RNA-sequencing. (A) After a consensus clustering of gene expression profiles, GSEA was performed between FX1-treated and DMSO-treated AML cells. Representative gene signatures are highlighted and sorted with biological functions shown at the right. (B-G) Enrichment plots of regulated gene signatures of interest obtained by GSEA analysis showing enrichments in FX1-treated vs DMSO. NES and FDR values are attached for each of the gene sets.