Figure 4.
Functional and gene expression analyses of Gpr56 downstream effectors. (A-C) Frequency of Venus+ cells (A) and CFU-C colonies of day 12 V+ sorted cells (B-C) treated with ROCK inhibitor (Y-27632) and collagen (Col3a1). (D) Spring analysis of AGM-derived HSPCs38 displays the differential expression of Gpr56, collagen (Col3a1), β arrestin (Arrb2), Gpr97, and Gpr114 in the 2 cell clusters (EC and HC1) of highly enriched HSPCs (CD31+G2VmedcKithi) from E10.5/11.5 AGM IAHC cells.

Functional and gene expression analyses of Gpr56 downstream effectors. (A-C) Frequency of Venus+ cells (A) and CFU-C colonies of day 12 V+ sorted cells (B-C) treated with ROCK inhibitor (Y-27632) and collagen (Col3a1). (D) Spring analysis of AGM-derived HSPCs38  displays the differential expression of Gpr56, collagen (Col3a1), β arrestin (Arrb2), Gpr97, and Gpr114 in the 2 cell clusters (EC and HC1) of highly enriched HSPCs (CD31+G2VmedcKithi) from E10.5/11.5 AGM IAHC cells.

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