Temporal activation of B cells determines phenotype. Summary data of (A) the proportion of B cells in the spleen and (B) the number of B cells in the spleen of LMC, mb1^DPWY, and CD21^DPWYmice. Data are from n = 5-17 mice per group and summary data are shown as the mean ± SEM of data collected from 3 independent experiments. (C) Representative plots of bone marrow B-cell development based on the Hardy classification system in LMC and CD21^DPWY mice. Data are representative of n = 9 LMC and n = 5 CD21^DPWY mice from 3 independent experiments. ***P < .001. NS, not significant.