Figure 4.
Sequential immunostaining reveals an increase in tissue-specific RARαhi CD8 effector T cells coexpressing T-bet and IL-23R in GI-GVHD. (A) RARαhi CD8 and (B) CD4 T-cell numbers in subcrypt regions of upper and lower GI biopsies from allo-HSCT patients with and without histologic GVHD and lower GI biopsies from healthy controls. (C) CD8 T-cell numbers co-expressing T-bet or (D) IL-23R in subcrypt regions of upper and lower GI biopsies from allo-HSCT patients with and without histologic GVHD and lower GI biopsies from healthy controls. (E) SPICE plots depicting subpopulations of CD8 T cells in subcrypt regions of GI biopsies. Arcs represent median frequencies of CD8 T cells expressing high RARα, T-bet, or IL-23R. Slices represent median frequencies of CD8 T-cell subpopulations by coexpression patterns of RARα, T-bet, and IL-23R. Results are shown for upper and lower GI biopsies from 4 healthy controls and 22 allo-HSCT patients, 15 with and 7 without histologic GVHD. (F) CD8 T-cell subpopulations expressed as a percentage of total CD8 cells in subcrypt regions of upper and lower GI biopsies from 4 healthy controls (circles) and 22 allo-HSCT patients, 15 with (diamonds) and 8 without (triangles) histologic GVHD. (G) Absolute numbers of CD8 T-cell subpopulations in subcrypt regions of GI biopsies. Color key is as shown in panel E. (H) SPICE plots depicting frequencies of subpopulations of CD8 T cells in epidermis of skin biopsies from allo-HSCT patients. Pie arcs and slices are designated as in panel E. Results are shown for biopsies from 11 allo-HSCT patients, 8 with and 3 without histologic skin GVHD. (I) Absolute numbers of RARαhi CD4 T cells coexpressing FOXP3 in subcrypt regions of GI biopsies from 12 allo-HSCT patients and 4 healthy controls. Numbers of RARαhi T-bet+ CD8 effector T cells in GI biopsies from allo-HSCT patients with histologic GVHD are shown for comparison. Horizontal lines and adjacent numbers are medians. *P < .05; **P < .01; ***P < .001, analysis of variance (ANOVA) tests with posttest correction for multiple comparisons.

Sequential immunostaining reveals an increase in tissue-specific RARαhi CD8 effector T cells coexpressing T-bet and IL-23R in GI-GVHD. (A) RARαhi CD8 and (B) CD4 T-cell numbers in subcrypt regions of upper and lower GI biopsies from allo-HSCT patients with and without histologic GVHD and lower GI biopsies from healthy controls. (C) CD8 T-cell numbers co-expressing T-bet or (D) IL-23R in subcrypt regions of upper and lower GI biopsies from allo-HSCT patients with and without histologic GVHD and lower GI biopsies from healthy controls. (E) SPICE plots depicting subpopulations of CD8 T cells in subcrypt regions of GI biopsies. Arcs represent median frequencies of CD8 T cells expressing high RARα, T-bet, or IL-23R. Slices represent median frequencies of CD8 T-cell subpopulations by coexpression patterns of RARα, T-bet, and IL-23R. Results are shown for upper and lower GI biopsies from 4 healthy controls and 22 allo-HSCT patients, 15 with and 7 without histologic GVHD. (F) CD8 T-cell subpopulations expressed as a percentage of total CD8 cells in subcrypt regions of upper and lower GI biopsies from 4 healthy controls (circles) and 22 allo-HSCT patients, 15 with (diamonds) and 8 without (triangles) histologic GVHD. (G) Absolute numbers of CD8 T-cell subpopulations in subcrypt regions of GI biopsies. Color key is as shown in panel E. (H) SPICE plots depicting frequencies of subpopulations of CD8 T cells in epidermis of skin biopsies from allo-HSCT patients. Pie arcs and slices are designated as in panel E. Results are shown for biopsies from 11 allo-HSCT patients, 8 with and 3 without histologic skin GVHD. (I) Absolute numbers of RARαhi CD4 T cells coexpressing FOXP3 in subcrypt regions of GI biopsies from 12 allo-HSCT patients and 4 healthy controls. Numbers of RARαhi T-bet+ CD8 effector T cells in GI biopsies from allo-HSCT patients with histologic GVHD are shown for comparison. Horizontal lines and adjacent numbers are medians. *P < .05; **P < .01; ***P < .001, analysis of variance (ANOVA) tests with posttest correction for multiple comparisons.

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