TSP-1^−/− and CD36^−/− display prolonged bleeding times after iloprost injection. (A) Whole blood was incubated alone or with PGI₂ (50 nM) for 2 minutes, then perfused at arterial shear 1000 s⁻¹ for 2 minutes over a collagen matrix (50 µg/mL). Images of adherent platelets were taken by fluorescence microscopy. (i) Representative images of arterial flow experiments, (ii) data presented as inhibition of surface coverage (%), mean ± standard error of the mean (N = 5; *P < .05, Mann-Whitney U test). (B) Whole blood from WT and TSP-1^−/− mice was labeled with DIOC6 and perfused over collagen (50 mg/mL) at a shear rate of 1000^-s for 2 minutes. The thrombi were subjected to a post-flow with Tyrode’s bovine serum albumin buffer with or without PGI₂ (50 nM) at 1000^-s for 4 minutes. (i) Representative images at the end of the postflow. (ii) Data are presented as reduction in surface coverage (%); N = 5 (P < .05). (C) WT, TSP-1^−/−, and CD36^−/− mice were transfused with iloprost (80 ng/mL) for 2 minutes or vehicle control, and bleeding time was examined by using the tail bleeding assay. Animals were counted after complete cessation of bleeding for 1 minute. Data are presented as scatter plot; every dot represents an individual animal, and the black line indicates mean (N = 9; **P < .01).