Artemisinins and SOR reduced MCL1 protein, and artemisinins elevated DDIT3/CHOP. MOLM14 and MV4;11 AML cells were incubated for 18 hours with DMSO, AS (10 μM), ART838 (200 nM), SOR (5 μM), or AraC (150 nM). (A) Western blotting was performed for MCL1 (brown circle), BCL2 (blue square), CHOP (green triangle), and housekeeping gene β-actin. Bar graphs summarize the relative fold-change of each protein level by densitometry in drug- vs DMSO-treated (represented by dotted line) cells; means of 3 independent experiments plus or minus SEM. (B) SYBR Green quantitative reverse transcription PCR was performed for MCL1 and DDIT3 mRNA. Cycle threshold values were normalized to housekeeping gene glyceraldehyde-3-phosphate dehydrogenase and fold-change relative to DMSO (represented by dotted line) was graphed; means of 3 independent experiments with triplicate samples plus or minus SEM. Statistical comparisons were performed for drug- vs vehicle (DMSO)-treated cells. *P < .05; **P < .01; ***P < .001; no asterisk if P > .05.