Figure 3.
Overexpression of sh-resistant AK2 isoform rescues T-ALL apoptosis. (A) Percentage of Annexin V and propidium iodide (Ann-PI)–negative cells at day 5 post-IPTG induction of JURKAT cells transduced by an inducible shAK2 vector. (B) Western blots of AK2 and AK1 in JURKAT and RPMI 8402 cell lines overexpressing 2 AK2 isoforms resistant to shAK2, AK2A, and AK2B with or without shAK2. (C-D) Absolute number of cells expressing either AK2A and AK2B isoforms or shAK2 or both in JURKAT (C) and RPMI 8402 (D) cell lines over time posttransduction. (E) Percentage of Ann-PI–positive cells expressing either AK2A and AK2B isoforms and shAK2 or shAK2 alone in JURKAT and RPMI 8402 cell lines every 2 days from day 2 (D2) post-shAK2 transduction to D8. All experiments were made in technical duplicates. ns, not significant. *P < .05; **P < .01; ***P < .001. ns, not significant. 

Overexpression of sh-resistant AK2 isoform rescues T-ALL apoptosis. (A) Percentage of Annexin V and propidium iodide (Ann-PI)–negative cells at day 5 post-IPTG induction of JURKAT cells transduced by an inducible shAK2 vector. (B) Western blots of AK2 and AK1 in JURKAT and RPMI 8402 cell lines overexpressing 2 AK2 isoforms resistant to shAK2, AK2A, and AK2B with or without shAK2. (C-D) Absolute number of cells expressing either AK2A and AK2B isoforms or shAK2 or both in JURKAT (C) and RPMI 8402 (D) cell lines over time posttransduction. (E) Percentage of Ann-PI–positive cells expressing either AK2A and AK2B isoforms and shAK2 or shAK2 alone in JURKAT and RPMI 8402 cell lines every 2 days from day 2 (D2) post-shAK2 transduction to D8. All experiments were made in technical duplicates. ns, not significant. *P < .05; **P < .01; ***P < .001. ns, not significant. 

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