Figure 4.
Proteomic comparison of control and GPS CD34+HSC-derived neutrophil culture. (A) Representative plots of the 3 isolated cell populations obtained by fluorescence-activated cell sorting based on cell surface markers CD11b and CD16 from control or GPS CD34+ HSC-derived cells at day 10 or 17 of the cell culture. (B) Stacked bar plots showing proportions of measured granule protein content between control (CTRL) PBMC CD34+ HSCs cultured CD16POS vs blood-derived PMNs (left panel) and GPS PBMC CD34+ HSCs cultured CD16POS vs blood-derived PMNs (right panel). (C) Comparisons among proteomes of the 3 different development stages (CD11bNEGCD16NEG, CD11bPOSCD16NEG, and CD11bPOSCD16POS) at the end of differentiation from the CD34+ HSC cultures derived from healthy controls or patients with GPS.

Proteomic comparison of control and GPS CD34+HSC-derived neutrophil culture. (A) Representative plots of the 3 isolated cell populations obtained by fluorescence-activated cell sorting based on cell surface markers CD11b and CD16 from control or GPS CD34+ HSC-derived cells at day 10 or 17 of the cell culture. (B) Stacked bar plots showing proportions of measured granule protein content between control (CTRL) PBMC CD34+ HSCs cultured CD16POS vs blood-derived PMNs (left panel) and GPS PBMC CD34+ HSCs cultured CD16POS vs blood-derived PMNs (right panel). (C) Comparisons among proteomes of the 3 different development stages (CD11bNEGCD16NEG, CD11bPOSCD16NEG, and CD11bPOSCD16POS) at the end of differentiation from the CD34+ HSC cultures derived from healthy controls or patients with GPS.

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