Characterization of thrombi from control and tumor-bearing mice. Thrombi were embedded in paraffin, sectioned longitudinally, and stained with hematoxylin and eosin (A) or Martius Scarlet Blue (B). With hematoxylin and eosin, red blood cell areas are red, platelet areas are pink, and inflammatory cell areas are purple. With Martius Scarlet Blue staining, red blood cell areas are yellow, platelet areas are gray, and fibrin areas are red. Representative pictures from 5 thrombi from controls and 6 thrombi from PANC-1 tumor-bearing mice. Scale bars, 700 µm. (C) Macrophages in thrombi from control and tumor-bearing mice were stained with an anti-Iba-1 antibody. Scale bars, 200 µm.