Loss of PPARγ function exacerbates PH and RVH in endothelial-targeted SS^ePPARγKOchimeric mice. PPARγ levels are decreased, whereas ET-1 and VCAM1 levels are increased in SS^ePPARγKO mouse lungs. The chimeric SS^ePPARγKO mice were generated by transplanting SCD bone marrow from Townes mice using previously published methods^50-53  into ePPARγKO mice as reported.^42,54  ePPARγKO mice were irradiated and transplanted with SS bone marrow. Six weeks after bone marrow transplant, following engraftment, the animals were studied. (A) RVSP was recorded in anesthetized mice with a pressure transducer. Each bar represents the mean RVSP in mm Hg ± SEM; n = 5 to 7. (B) The ratio of the weight of the RV to the LV + septum [RV: (LV + S)] is presented as an index of RVH; n = 5 to 7. (C-E) Whole lung homogenates were collected from littermate control (AA^LitCon and SS^LitCon) and chimeric (AA^ePPARγKO and SS^ePPARγKO) mice. Real-time qPCR was performed on lung tissue. Lung PPARγ (C), ET-1 (D), or VCAM (E) levels are expressed relative to 9S normalized to CON values. Each bar represents the mean ± SEM. *P < .05 vs AA^LitCon; ⁺P < .05 vs SS^LitCon, n = 3 to 7.