Overexpression of Chmp5 ameliorates defects in late-stage terminal erythropoiesis in mDia2-deficient mice. (A) Outline of the transplantation strategy. (B) RBC count and hemoglobin levels in mice receiving the indicated bone marrow cells for 2 months. (C) Spleen weight of mice in panel B. (D) Morphologic analyses of bone marrow smears from mice in panel B. Arrows indicate binucleated orthochromatic erythroblasts. Bar represents 20 μm. Flow cytometric assays in the bottom panels quantify the percentage of S/G2/M phases reflecting the number of binucleated cells. (E) Quantitative analysis of panel D. (F) Lineage-negative cells from the bone marrow of the indicated recipient mice were cultured in erythropoietin-containing medium for 2 days. Flow cytometric analyses were performed with the indicated parameters to identify proteins on the reticulocytes. *P < .05; **P < .01; ***P < .001.