mDia2 is involved in membrane remodeling and organelle clearance during terminal erythropoiesis. (A) Lineage-negative cells from the bone marrow of the indicated mice were cultured in erythropoietin-containing medium for 2 days. Flow cytometric analysis was performed to determine the surface levels of the indicated proteins, DNA, and forward scatter (FSC) in wild-type and mDia2-deficient reticulocytes. (B) Same as panel A except the cells were also stained with lysosome tracker and MitoTracker Red for the detection of lysosome and mitochondria content, respectively. (C-D) Fluorescence analyses of the indicated organelles in the orthochromatic stages of the erythroblasts (C) and reticulocytes (D) in the cells from panel B.