Gpr124 was present in glomeruloid bodies of E13.5 Tfpi^−/−brain. Forebrain sections from Tfpi^+/+ and Tfpi^−/− littermates were stained for the extracellular domain of Gpr124 (red) and thrombomodulin (green), with DAPI-stained nuclei (blue) to depict the PNVP of the anterior forebrain and the adjacent lateral ventricle. Gpr124 stains well-organized vasculature in the Tfpi^+/+ brain and the disorganized vasculature penetrating from the PNVP into the brain parenchyma in the Tfpi^−/− brain. In the overlay, thrombomodulin stains the endothelial cells in the same pattern as Gpr124 in both embryos. Autofluorescence of the embryonic nucleated RBCs is observed within the vasculature of both embryos and areas of hemorrhage in the Tfpi^−/− embryo (scale bars, 30 µm).