Pericytes and microglial cells were present within glomeruloid bodies in the E15.5 Tfpi^−/−brain. (A) Immunofluorescence of brain vasculature from littermates stained for PDGFR-β (red) and thrombomodulin (green), with DAPI-stained nuclei (blue). Occasional pericytes (red) were present along vasculature within the Tfpi^+/+ brain parenchyma. The glomeruloid bodies within the Tfpi^−/− embryo had numerous pericytes associated with the aggregated endothelial cells (scale bars, 10 µm). (B) Immunofluorescence of brain vasculature from littermates stained for ionized calcium-binding adapter molecule 1 (IBA1; red/yellow) and thrombomodulin (green), with DAPI-stained nuclei (blue). IBA1-expressing microglial cells were associated with endothelial cells in Tfpi^+/+ and Tfpi^−/− embryos, with no discernible differences between genotypes (scale bars, 10 µm).