Figure 1.
CCR7L deficiency prolongs the intrathymic dwell time Of neonatal SP thymocytes. (A-C) Flow cytometric analysis of thymocytes in plt/plt (n = 16) and +/plt (n = 16) littermate postnatal day–10 neonatal mice. cSP4 were gated as CD4+TCRβhiCD25−Foxp3−, SP8 as CD8+TCRβhi, immature SP as CD69+CD62L−, and mature SP as CD69−CD62L+. Percentages of cSP4 and SP8 expressing Ki67 are also shown in panels B and C. (E-D) Rag2GFP levels in cSP4 and SP8 thymocytes from Rag2GFPplt/plt mice (n = 5; red) and Rag2GFP+/plt controls (n = 7; blue). Gray histograms indicate nonfluorescent control cells. (F) Numbers and frequencies of M2a, M2b, and M2c subsets of mature CD69−CD62L+ cSP4 and SP8 in Rag2GFP+/plt (n = 16) controls and Rag2GFP plt/plt mice (n = 16). Bar chart indicates percentages of each subset in Rag2GFP+/plt (blue bars) and Rag2GFP plt/plt (red bars). (G) Rag2GFP levels in the M2c subset of cSP4 and SP8 thymocytes from +/plt controls (n = 7; blue) and plt/plt (n = 5; red) mice. Bar charts show percentages of M2a, M2b, and M2c subsets of cSP4 and SP8 in +/plt (blue bars) and plt/plt (red bars). For analysis of data in panels F and G, multiple comparison analysis was achieved by a 2-way analysis of variance followed by Sidak’s posttest in GraphPad Prism to determine statistical differences. In all cases, error bars represent mean ± SEM. Flow cytometric data representative of at least 3 independent experiments. **P < .01, ***P < .001, ****P < .0001. DP, double positive; MFI, mean fluorescence intensity.

CCR7L deficiency prolongs the intrathymic dwell time Of neonatal SP thymocytes. (A-C) Flow cytometric analysis of thymocytes in plt/plt (n = 16) and +/plt (n = 16) littermate postnatal day–10 neonatal mice. cSP4 were gated as CD4+TCRβhiCD25−Foxp3−, SP8 as CD8+TCRβhi, immature SP as CD69+CD62L−, and mature SP as CD69−CD62L+. Percentages of cSP4 and SP8 expressing Ki67 are also shown in panels B and C. (E-D) Rag2GFP levels in cSP4 and SP8 thymocytes from Rag2GFPplt/plt mice (n = 5; red) and Rag2GFP+/plt controls (n = 7; blue). Gray histograms indicate nonfluorescent control cells. (F) Numbers and frequencies of M2a, M2b, and M2c subsets of mature CD69−CD62L+ cSP4 and SP8 in Rag2GFP+/plt (n = 16) controls and Rag2GFP plt/plt mice (n = 16). Bar chart indicates percentages of each subset in Rag2GFP+/plt (blue bars) and Rag2GFP plt/plt (red bars). (G) Rag2GFP levels in the M2c subset of cSP4 and SP8 thymocytes from +/plt controls (n = 7; blue) and plt/plt (n = 5; red) mice. Bar charts show percentages of M2a, M2b, and M2c subsets of cSP4 and SP8 in +/plt (blue bars) and plt/plt (red bars). For analysis of data in panels F and G, multiple comparison analysis was achieved by a 2-way analysis of variance followed by Sidak’s posttest in GraphPad Prism to determine statistical differences. In all cases, error bars represent mean ± SEM. Flow cytometric data representative of at least 3 independent experiments. **P < .01, ***P < .001, ****P < .0001. DP, double positive; MFI, mean fluorescence intensity.

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