Adult lung MKs exhibit higher ploidy and express markers of maturation. (A) Ploidy analysis of CD41a⁺ MKs demonstrated a greater proportion of high ploidy MKs from the lung. (B-C) Flow cytometric assessment showed that a greater proportion of lung MKs was positive for the key maturation marker CD42d, which was significant across 3 biological replicates. BSA enrichment was used for these experiments. Ploidy analysis without BSA enrichment was also performed and showed similar results (supplemental Figure 5). "X" and "●" represent individual data points, whereas the horizontal bars are averages. *P < .05.