SLE murine model expressing human FcγRIIA. (A) Whole body sagittal-section of a B/WF1.FcγRIIA^TGN mouse (female, 28 weeks) with SLE showing FcγRIIA staining in CD11b⁺ cells (white arrows) and in CD41⁺ megakaryocytes (white arrow heads) in the brain (b), lymph node (c), salivary gland (d), lungs (e), liver (f), kidney (g), intestine (h), and bone marrow (i). Scale bars, 5 mm (A); 50 µm (b-i). The illustrated image is a composite of all scanned tissue areas automatically generated. Areas not presenting tissues were automatically filled in black to generate a clearer image. (B) Levels of total serum IgG in B/WF1.FcγRIIA^Null and B/WF1.FcγRIIA^TGN female mice from 10 to 30 weeks. N = 5 to 8 per group and per time point. (C) Plasma B cells in the spleen of B/WF1.FcγRIIA^Null and B/WF1.FcγRIIA^TGN mice at 28 weeks of age (n = 5). Data are presented as the mean ± SEM. Statistical analyses: 2-way analysis of variance. (B) Šídák’s multiple comparisons test for comparing B/WF1.FcγRIIA^Null and B/WF1.FcγRIIA^TGN, then Dunnett’s multiple comparisons test to compare each strain to 10 weeks. (C) Mann-Whitney U test (comparing B/WF1.FcγRIIA^Null and B/WF1.FcγRIIA^TGN). *P < .05, **P < .01.