CITE-seq analyses confirm markers for naïve and lineage-committed progenitor cells. (A) tSNE visualization of the CITE-seq analysis of days 10 and 13 CD235a⁻ suspension cells. Dimension reduction and clustering analyses were performed using only transcriptomic data (hiPSC-SFCi55). (B) tSNE visualization of the libraries obtained from CD235a⁻ suspension cells collected at days 10 and 13 showing the direction of lineage commitment. (C) Single-cell protein expression level of the membrane markers associated with the different cell types. Data are visualized on tSNE. (D) tSNE plot and annotation of clustering obtained from analysis derived from ADT data alone. (E) Visualization of the libraries obtained from CD235a⁻ suspension cells projected on the tSNE obtained from ADT data in panel D. Cell progression shows lineage commitment trajectory, with cells colored according to the day of collection. (F) Confusion matrix of clustering obtained from complete transcriptomic data (RNA) and that were obtained from ADT data alone. The color of each box indicates the percentage of cells classified in each RNA vs ADT cluster. (G) Gene expression levels of human fetal marker genes in the naïve and lineage-committed progenitors. tSNE, t-distributed stochastic neighbor embedding.