Trajectory analyses support naïve progenitor identity and their progression to lineage-committed progenitors. (A) Diffusion plot displays the naïve progenitors in the core region of the plot from which the 3 directions of commitment originate; the arrows indicate the commitment directions. (B) Representation of each cluster on the diffusion plot. (C) PAGA analysis showed that naïve clusters were connected to the lineage-committed cells. Each node contains a pie chart showing the proportion of cells for each cluster. Colors indicate cluster identities. (D) Monocle trajectory analyses demonstrates a pattern similar to that obtained from the diffusion plot shown in panel A, with naïve progenitors at the top of the hierarchy, with progression toward committment. (E) Monocle trajectory visualizing each cluster individually. (F) Expression levels of the marker genes coding for transcription factors associated with each cluster; bar color indicates the cluster.