Figure 5.
Flow cytometry to identify progeny of GFP- or YFP-transduced CD34+cells transplanted via either IB or IV routes, respectively. Fluorescence-activated cell sorting plots showing: gating by forward (FSS) and side scatter (SSC) to identify cell types (A), separation of GFP+ and YFP+ granulocytes from a control RM previously transplanted IV with CD34+ cells transduced with equal numbers of both vectors (B), and proportions of circulating granulocytes deriving from each graft (IV and OIB) in 3 RMs at 6, 9, and 12 days posttransplantation (C).

Flow cytometry to identify progeny of GFP- or YFP-transduced CD34+cells transplanted via either IB or IV routes, respectively. Fluorescence-activated cell sorting plots showing: gating by forward (FSS) and side scatter (SSC) to identify cell types (A), separation of GFP+ and YFP+ granulocytes from a control RM previously transplanted IV with CD34+ cells transduced with equal numbers of both vectors (B), and proportions of circulating granulocytes deriving from each graft (IV and OIB) in 3 RMs at 6, 9, and 12 days posttransplantation (C).

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