Differential miR34a-MYC-PDL1 expression in TP53 mutant vs wild-type MDS/sAML. Relative MYC expression as measured by quantitative polymerase chain reaction (qPCR) in the MCC cohort (A) and validation cohort (B). (C) Correlation of MYC expression with the percent of HSCs expressing cell surface PDL1 in the MCC cohort. (D) Volcano plot highlighting differential miRNA (false discovery rate <1) expression in patients with TP53 mutations vs the wild-type gene. Relative MYC (E) and miR-34a-5p (F) expression in isogenic TP53 knockout (KO) and missense mutant cell lines. Error bars represent standard error of 3 replicates.