Expression of kit receptors in the zebrafish embryonic development. (A) qPCR expression analysis of kita (left panel) and kitb (right panel) at different developmental stages. Data were normalized using ef1a as the housekeeping gene and relative to the mean of 24-hpf sample (relative expression level 1). (B) Sorting strategy used for FACS isolation of gata1:DsRed⁺ cells (upper left panel) from transgenic zebrafish at 24 hpf. qPCR expression analysis of gata1 (upper right panel), kita (lower left panel), and kitb (lower right panel) in gata1:DsRed⁻ and gata1:DsRed⁺ cells. Data were normalized using ef1a as the housekeeping gene and relative to the mean of gata1:DsRed⁺ 24-hpf sample (relative expression level 1). (C) Sorting strategy used for FACS isolation of lcr:EGFP⁺ cells (upper left panel) from transgenic zebrafish at 24 hpf. qPCR expression analysis of gata1 (upper right panel), kita (lower left panel), and kitb (lower right panel) in lcr:EGFP⁻ and lcr:EGFP⁺ cells. Data were normalized using ef1a as the housekeeping gene and relative to the mean of lcr:EGFP⁺ 24-hpf sample (relative expression level 1).