GPX4 had a perinuclear distribution during enucleation and localized with lipid raft markers flotillin and cholera toxin b subunit. (A) Representative image of AlexaFluor 647–conjugated GPX4 immunolabeling and DAPI staining during erythroblast enucleation (n = 3). These images were taken at day 15 of differentiation at 60× original magnification used with oil immersion. (B-C) Representative image of DAPI staining and AlexaFluor 647–conjugated GPX4 and AlexaFluor 488–conjugated Flotillin-2 immunolabeling (B) or AlexaFluor 647–conjugated GPX4 and AlexaFluor 488–conjugated CTB immunolabeling (C) on day 15 at the beginning of enucleation at 60× magnification used with oil immersion (n = 3).